Associate Professor

Department:
Health and Human Performance

Exercise Science

Office Number: 104D GAR

Phone/Fax:
(713) 743-9854 , (713) 743-9860  (Fax)

Email: mclarke@uh.edu

Home Page:
http://grants.hhp.coe.uh.edu/mclarke/index.htm

Research Interests:

Muscle physiology, muscle adaptation to mechanical loading, cellular basis of muscle function in health and disease.

Recently Funded Grant Proposals

NASA-NRA-HRP 2007: Monitoring of Biomarkers of Bone Loss in Human Sweat – a non-invasive, time efficient means of monitoring bone resorption markers under micro and partial gravity loading conditions. M. S. F. Clarke, Ph.D. (PI); D.P. O’Connor, Ph.D. (Co-I). (Total Amount: $719,200).

NIA/NIH R03 Program: “TLR4 Signaling: A Link between Obesity and Chronic Inflammation.” (McFarlin B.K., Jackson, A.S., Clarke, M.S.F. and Bray, M.S.; Role in Project: Co-Investigator) (Total Amount: $150,000)

ISSO Postdoctoral Fellowship Program (2004-2007): Subtractive Proteomic Profiling of Control, Atrophied and Protected Rat Skeletal Muscle by Dynamic Foot Stimulation (DFS). (Clarke, M.S.F, Franchek, M and Feeback, D.L.; Role in Project: Principal Investigator) (Total Amount - $120,000).

NASA-Johnson Space Center Research Grant Program “Development and Characterization of a Three-Dimensional Tissue Culture Model of Bone” (Clarke, M.S.F.; Role in Project: Principal Investigator) (2004-2006) (Total Amount - $94,932).

Recently Submitted Grant Projects

NIH-NIAMS-RO3 2008 Circulating Markers of Exercise-Induced Muscle Damage in Humans M. S. F. Clarke, Ph.D. (PI); D.P. O’Connor, Ph.D. (Co-I). (Total Funds requested $149,000).

Recent Publications:

Journal Articles

Gazda, D.B., Schultz, J.R., Clarke, M.S.F. (2008) Liquid Metering Centrifuge Sticks (LMCS):  A Centrifugal Approach to Metering Known Sample Volumes for Colorimetric Solid Phase Extraction (C-SPE). SAE Transactions-Journal of Aerospace (in press).

Jones, J.J., Riggs, P.K., Yang, T.C., Pedemonte, C.H., Clarke, M.S.F., Feeback, D.L. and Au, W.A. (2007) Ionizing radiation-induced bioeffects in space and strategies to reduce cellular injury and carcinogenesis. Aviation, Space and Environmental Medicine 78 (4): 67-78.

Hutchison, A.T. and Clarke, M.S.F. (2006) An Isometric Strength Testing Device for Use with the Elderly: Validation Compared with Isokinetic Measures. Physical and Occupational Therapy in Geriatrics. 25(2):1-12.

Sotiriadou, S. Kyparos,A., Albani,M., Arsos,G., Clarke,M.S.F., Sidiras, G., Angelopoulou, N. and Matziari, C. (2006) Soleus muscle force following downhill running in ovariectomized rats treated with estrogen. Applied Physiology, Nutrition and Metabolism (formerly Canadian JAP) 31 (4): 449-59.

Kyparos,A., Feeback, D.L., Layne, C.S., Martinez, D.A. and Clarke, M.S.F. (2005) Mechanical stimulation of the plantar foot surface attenuates soleus muscle atrophy induced by hindlimb unloading in rats. J. App. Physiol. 99:739-46. Epub 2005 Apr 7.

Singh, V., Desta, Y., Datta, P., Guy, J., Goettert, J., Clarke, M.S.F. and Feeback, D.L. (2005) “A Hybrid Approach for Fabrication of Polymeric BioMems Devices.” Microsystem Technologies, 13 (3): 396-377.

Carterson AJ, Honer zu Bentrup K, Ott CM, Clarke MSF, Pierson DL, Vanderburg CR, Buchanan KL, Nickerson CA, Schurr MJ. (2005) A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis. Infection & Immunity. 73(2):1129-40.

Clarke, M.S.F., Vanderburg, C.R. and Feeback, D.L. (2002) The effect of acute microgravity upon mechanically-induced membrane damage and membrane-membrane fusion. J. Grav. Physiol.,8:37-47.

Nickerson, C.A., Goodwin, T., Terlonge, J., Ott, C. Mark, Clarke, M.S.F., Buchanan, K.L., Uicker, W.C., Emami, K., Cedor, C.L., Ramamurthy, R., Hammond, T. and Pierson, D.L. (2001) Novel Tissue Assemblies:Models for Enteric Pathogenesis. Infection & Immunity. 69:7106-7120.

Clarke, M.S.F., Vanderburg, C.R., Bamman, M.M., Caldwell, R.W. and Feeback, D.L. (2000) In situ localization of cholesterol in skeletal muscle using a monoclonal antibody. J. Appl. Physiol. 89: p731-741.

Clarke CH, Norfleet AM, Clarke MSF, Watson CS, Cunningham KA and Thomas ML. (1999) Peri-membrane localization of the estrogen receptor a protein in neuronal processes of cultured hippocampal neurons. Neuroendocrinology 71:34-42.

Clarke, M.S.F., Prendergast, M.A. and Terry, A.V. (1999) Plasma membrane ordering agent Pluronic F-68 (PF-68) reduces neurotransmitter uptake and release and produces learning and memory deficits in rats. Learning & Memory 6: 634-649.

Bamman, M.M., Clarke, M.S.F., Talmadge, R.J. and Feeback, D.L. (1999) Enhanced protein electrophoresis technique for separating human skeletal muscle myosin heavy chain isoforms. Electrophoresis 20: 466-468.

Clarke, M.S.F., Bamman, M.M. and Feeback, D.L. (1998) Bed-rest decreases mechanically induced myofiber wounding and consequent wound-mediated FGF release. J. Appl. Physiol. 85: 593-600.

Bamman, M.M., Clarke, M.S.F., Feeback, D.L., Talmadge, R.J., Stevens, B.R., Leiberman, S.A. and Greenisen, M.C. (1997) Impact of resistance exercise during bed rest on skeletal muscle sarcopenia and myosin isoform  distribution. J. Appl. Physiol. 84:157-163.

Clarke, M.S.F. and Feeback, D.L. (1996) Mechanical load induces sarcoplasmic wounding and FGF release in differentiated human skeletal muscle cells. The FASEB Journal  10: 502-509.

Clarke, M.S.F., Pritchard, K.A., Medows, M. S. and McNeil, P.L. (1995) An atherogenic level of native LDL increases endothelial cell vulnerability to shear-induced plasma membrane wounding and consequent release of basic fibroblast growth factor. Endothelium  4: p127-139.

Clarke, M.S.F., Caldwell, R.W., Chiao, H., Miyake, K. and McNeil, P.L. (1995) Contraction-induced cell wounding and release of fibroblast growth factor in rat heart. Circulation Research 76; 927-934.

Clarke, M.S.F., Vanderburg, C.R., Hay, E.D. and McNeil, P.L. (1994) Cytoplasmic loading of dyes, protein and plasmid DNA using an impact-mediated procedure. Biotechniques 17 (6); p1118-1125.

Terry, A.V. and Clarke, M.S.F. (1994) Nicotine stimulation of nerve growth factor receptor expression. Life Sciences 55; PL 91-98.

Clarke, M.S.F., Khakee, R. and McNeil, P.L. (1993) Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle.  J. Cell Science 106; p121-133.

Clarke, M.S.F. and McNeil, P.L. (1992) Syringe loading introduces macromolecules into living mammalian cell cytosol. J. Cell Science 102; p533-541.

Clarke, M.S.F. and West, D.C. (1991). The identification of proliferation and tumor-induced proteins in human endothelial cells: A possible target for tumor therapy. Electrophoresis 12; p500-508.

Clarke, M.S.F., Kiff, R.S., Kumar, S., Kumar, P. and West, D.C. (1991). The identification of proliferation related antigens in human endothelial cells as a possible target in tumor therapy. Int. J. Radiat. Biol. 60; p17-23.

Invited Reviews

Clarke, M.S.F. (2004) Effects of exercise on skeletal muscle in the aged. J. Musculoskeletal and Neuron Interactions. 4: 175-178.

Book Chapters

Feeback, D.L. and Clarke, M.S.F. (2008) Space flight induced changes in muscle structure and function. In  Space Physiology and Medicine ed. A.E. Nicogossian, S.L. Pool & C.L. Huntoon (4th Edition) Lippincott, Williams and Wilkins (in press).

Clarke, M. S. F., Jones, J.A. and Feeback, D.L. (2006) Syringe Loading: a method for assessing plasma membrane function as a reflection of the mechanically-induced membrane wound response. In. Cell Biology: A Laboratory Handbook. ed. J.E. Celis (3rd Edition), Academic Press. pp 233-240.

Clarke, M.S.F., Feeback, D.L. and Vanderburg, C.R. (2006). Impact-mediated loading of large macromolecules into adherent mammalian cells In. Cell Biology: A Laboratory Handbook. ed. J.E. Celis (3rd Edition), Academic Press. pp5-11.

Clarke, M.S.F., Weinberger, S. and Clarke, C.H. (2002) Detection of protein biomarkers during oxidative stress using Surface Enhanced Laser Desorption/Ionization-Time of Flight (SELD-TOF) Mass Spectrometry. In Oxidative Stress and Aging: Advances in Basic Science, Diagnostics and Intervention  ed. R.G. Cutler & H. Rodriguez, World Scientific Publishing Co. pp366-379.

McNeil, P.L., Clarke, M.S.F., and Miyaki, K. (1999). Cell wound assays. In Current Protocols in Cell Biology (J.S. Bonifacino, M. Dasso, J.B. Harford, J. Lippincott-Schwartz, and K.M. Yamada, eds.) pp 12.4.1-12.4.15, John Wiley & Sons. New York.

Clarke, M.S.F. and McNeil, P.L. (1998). Syringe Loading: a mechanically-based method for inserting macromolecules into cells. In. Cell Biology: A Laboratory Handbook. ed. J.E. Celis (2nd Edition), Academic Press. Volume 4. pp. 47 -54.

Clarke, M.S.F. and McNeil, P.L. (1994). Syringe Loading: a method for inserting macromolecules into cells in suspension. In. Cell Biology: A Laboratory Handbook. ed. J.E. Celis, Academic Press. pp. 30-36.

Patents and Intellectual Property

New Invention: Centrifuge Operated Slide Stainer (COSST)
(Clarke, M.S.F. and Feeback, D.L. , US Patent # 6008009).

New Invention: Unidirectional Cell Stretching Device  (Clarke, M.S.F. and Feeback, D.L., US Patent # 6107081)

New Invention: Method and Apparatus for Cytoplasmic Loading Using An Impact-Mediated Procedure – The G-Loader. (Clarke, M.S.F. et al., US Patent # 6221666).

New Invention: Directional Acceleration Vector Driven Displacement of Fluids (DAVD-DOF) (Clarke, M.S.F. and Daniel L. Feeback., US Patent # 6743632).

New Invention: Production of stable aqueous dispersions of carbon nanotubes  (Clarke, M.S.F. and Daniel L. Feeback, US Patent # 6878361).

New Invention: Spatial localization of dispersed single walled carbon nanotubes into useful structures  (Clarke, M.S.F.  US Patent # 6896864).

New Invention: Micro-fabricated volumetric capillary arrays for non-invasive collection of biological fluids. (Clarke, M.S.F. and Feeback, D.L.), Provisional Patent filed 3/05, status pending).

New Invention: Three-dimensional tissue constructs of human bone. (Clarke, M.S.F. et al.), Provisional Patent filed 4/07, status pending).

Technical Reports

Elevated Skin Blood Flow Influences Near Infrared Spectroscopy Measurements During Supine Rest. Lee, S.M.C. and Clarke, M.S.F. NASA Technical Report NASA/TP-2004-213149, S-942.

Compact, Automated Centrifugal Slide-Staining System for Use in Microgravity. Clarke, M.S.F. and Feeback, D.L., NASA Tech Briefs, June 2004.

Using Dynamic Foot Pressure as a Countermeasure to Muscle Atrophy (Final Report). Layne, C.S., Clarke, M.S.F., Martinez, D.A., Feeback, D.L. and Kyparos, A. UH Institute for Space Systems Operations Y2003 Annual Report, pages 66-73. (Dr. Clarke serves as a UH Co-PI along with Dr. Layne and Martinez on this project).

Improved Unidirectional Cell-Stretching Device. (MSC-22834) Clarke, M.S.F. and Feeback, D.L., NASA Tech Briefs, August 2002.

Slide-Staining System for Microgravity or Gravity. (MSC-22949) Clarke, M.S.F. and Feeback, D.L., NASA Tech Briefs, Jan 2001.

Education:

M.I. in BIOL. (Pharmacology), Manchester Metropolitan University, Manchester, England, 1991.
Ph.D. in Cell Biology and Biochemistry, Manchester Metropolitan University, Manchester, England, 1991.